Human anti-rhesus D IgG1 antibody produced in transgenic plants

Thomas Bouquin; Mads Thomsen; Leif Kofoed Nielsen; Trine Hefsgaard Green; John Mundy; Morten Hanefeld Dziegiel

doi:10.1023/A:1015226418688

Human anti-rhesus D IgG1 antibody produced in transgenic plants

Thomas Bouquin
, Mads Thomsen
, Leif Kofoed Nielsen
, Trine Hefsgaard Green
, John Mundy
, Morten Hanefeld Dziegiel

Publikation: Bidrag til tidsskrift › Tidsskriftsartikel › Forskning › peer review

Abstract

Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

Originalsprog	Engelsk
Tidsskrift	Transgenic Research
Vol/bind	11
Udgave nummer	2
Sider (fra-til)	115-122
Antal sider	8
ISSN	0962-8819
DOI	https://doi.org/10.1023/A:1015226418688
Status	Udgivet - apr. 2002
Udgivet eksternt	Ja

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title = "Human anti-rhesus D IgG1 antibody produced in transgenic plants",

abstract = "Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.",

keywords = "Agglutination Tests, Arabidopsis, Blotting, Western, Cloning, Molecular, Enzyme-Linked Immunosorbent Assay, Genetic Vectors, Humans, Immunoglobulin G, Monocytes, Plants, Genetically Modified, Recombinant Proteins, Respiratory Burst, Rh-Hr Blood-Group System, Journal Article, Research Support, Non-U.S. Gov't",

author = "Thomas Bouquin and Mads Thomsen and Nielsen, \{Leif Kofoed\} and Green, \{Trine Hefsgaard\} and John Mundy and Dziegiel, \{Morten Hanefeld\}",

note = "Funding Information: This work was funded by a EU BioMed Contract, BMH4-CT96-1545, NOVO Nordic Research Foundation to MHD, LKN and THG, and by the Danish Agricultural and Veterinary Research Council to TB.",

year = "2002",

month = apr,

doi = "10.1023/A:1015226418688",

language = "English",

volume = "11",

pages = "115--122",

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TY - JOUR

T1 - Human anti-rhesus D IgG1 antibody produced in transgenic plants

AU - Bouquin, Thomas

AU - Thomsen, Mads

AU - Nielsen, Leif Kofoed

AU - Green, Trine Hefsgaard

AU - Mundy, John

AU - Dziegiel, Morten Hanefeld

N1 - Funding Information: This work was funded by a EU BioMed Contract, BMH4-CT96-1545, NOVO Nordic Research Foundation to MHD, LKN and THG, and by the Danish Agricultural and Veterinary Research Council to TB.

PY - 2002/4

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N2 - Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

AB - Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

KW - Agglutination Tests

KW - Arabidopsis

KW - Blotting, Western

KW - Cloning, Molecular

KW - Enzyme-Linked Immunosorbent Assay

KW - Genetic Vectors

KW - Humans

KW - Immunoglobulin G

KW - Monocytes

KW - Plants, Genetically Modified

KW - Recombinant Proteins

KW - Respiratory Burst

KW - Rh-Hr Blood-Group System

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

UR - https://www.scopus.com/pages/publications/0036000252

U2 - 10.1023/A:1015226418688

DO - 10.1023/A:1015226418688

M3 - Journal article

C2 - 12054345

SN - 0962-8819

VL - 11

SP - 115

EP - 122

JO - Transgenic Research

JF - Transgenic Research

IS - 2

ER -

Human anti-rhesus D IgG1 antibody produced in transgenic plants

Abstract

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