The PEF family proteins sorcin and grancalcin interact in vivo and in vitro

Christian Hansen; Svetlana Tarabykina; Jonas M. La Cour; Karsten Lollike; Martin W. Berchtold

doi:10.1016/S0014-5793(03)00518-0

The PEF family proteins sorcin and grancalcin interact in vivo and in vitro

Christian Hansen, Svetlana Tarabykina, Jonas M. La Cour, Karsten Lollike, Martin W. Berchtold

Publikation: Bidrag til tidsskrift › Tidsskriftsartikel › Forskning › peer review

Abstract

The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.

Originalsprog	Engelsk
Tidsskrift	FEBS Letters
Vol/bind	545
Udgave nummer	2-3
Sider (fra-til)	151-154
Antal sider	4
ISSN	0014-5793
DOI	https://doi.org/10.1016/S0014-5793(03)00518-0
Status	Udgivet - 19 jun. 2003
Udgivet eksternt	Ja

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title = "The PEF family proteins sorcin and grancalcin interact in vivo and in vitro",

abstract = "The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.",

keywords = "Apoptosis, EF hand calcium binding protein, Grancalcin, Penta-EF hand family protein, Protein-protein interaction, Sorcin",

author = "Christian Hansen and Svetlana Tarabykina and \{La Cour\}, \{Jonas M.\} and Karsten Lollike and Berchtold, \{Martin W.\}",

year = "2003",

month = jun,

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doi = "10.1016/S0014-5793(03)00518-0",

language = "English",

volume = "545",

pages = "151--154",

journal = "FEBS Letters",

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TY - JOUR

T1 - The PEF family proteins sorcin and grancalcin interact in vivo and in vitro

AU - Hansen, Christian

AU - Tarabykina, Svetlana

AU - La Cour, Jonas M.

AU - Lollike, Karsten

AU - Berchtold, Martin W.

PY - 2003/6/19

Y1 - 2003/6/19

N2 - The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.

AB - The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.

KW - Apoptosis

KW - EF hand calcium binding protein

KW - Grancalcin

KW - Penta-EF hand family protein

KW - Protein-protein interaction

KW - Sorcin

UR - https://www.scopus.com/pages/publications/0037526593

U2 - 10.1016/S0014-5793(03)00518-0

DO - 10.1016/S0014-5793(03)00518-0

M3 - Journal article

C2 - 12804766

AN - SCOPUS:0037526593

SN - 0014-5793

VL - 545

SP - 151

EP - 154

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

The PEF family proteins sorcin and grancalcin interact in vivo and in vitro

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