Abstract
Background: Repeatedly freezing and thawing of samples can affect the stability of biomarkers in plasma samples. There is a lack of studies reporting how these preanalytical factors affect the stability of diabetes-related metabolic biomarkers. Therefore, the aim of the study was to investigate the effects of repeated freeze/thaw cycles (FTC) on the analysis of insulin, c-peptide, glucagon, total glucagon like peptide-1 (GLP-1), total glucose-dependent insulinotropic polypeptide (GIP), leptin and polypeptide YY (PYY).
Material and Methods: Plasma was prepared from blood samples collected from 10 healthy individuals. Each plasma sample was divided into 3 aliquots. An aliquot from each sample was analyzed immediately after preparation. The remaining aliquots were exposed to 3 and 5 repeated FTC. Samples were measured using a MESO Quickplex SQ 120 from Meso Scale Diagnostics LLC. (MSD) and the U-Plex Diabetes Combi 1 (hu) panel kit from MSD.
Results: The concentrations of GIP, GLP-1, insulin and PYY were statistical significantly affected by repeated FTC. After 5 FTC, the concentration of GIP was increased by 44 %, GLP-1 by 35 % and PYY by 22 %. There were no significant changes in the concentrations of glucagon, c-peptide and leptin after repeated FTC.
Conclusions: GIP, GLP-1 and PYY were significantly affected by repeated FTC. The concentration of these markers increased by 22-44 % with repeated FTC. Hence, repeated FTC can cause
significant changes in the concentrations of the biomarkers. Our results suggest that caution should be given when comparing biomarkers between plasma samples that have been subject to FTC.
Material and Methods: Plasma was prepared from blood samples collected from 10 healthy individuals. Each plasma sample was divided into 3 aliquots. An aliquot from each sample was analyzed immediately after preparation. The remaining aliquots were exposed to 3 and 5 repeated FTC. Samples were measured using a MESO Quickplex SQ 120 from Meso Scale Diagnostics LLC. (MSD) and the U-Plex Diabetes Combi 1 (hu) panel kit from MSD.
Results: The concentrations of GIP, GLP-1, insulin and PYY were statistical significantly affected by repeated FTC. After 5 FTC, the concentration of GIP was increased by 44 %, GLP-1 by 35 % and PYY by 22 %. There were no significant changes in the concentrations of glucagon, c-peptide and leptin after repeated FTC.
Conclusions: GIP, GLP-1 and PYY were significantly affected by repeated FTC. The concentration of these markers increased by 22-44 % with repeated FTC. Hence, repeated FTC can cause
significant changes in the concentrations of the biomarkers. Our results suggest that caution should be given when comparing biomarkers between plasma samples that have been subject to FTC.
| Original language | English |
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| Publication date | 25 Sept 2021 |
| Publication status | Published - 25 Sept 2021 |