Quality assessment of a placental perfusion protocol

Line Mathiesen, Tina Mose, Thit Juul Mørck, Jeanette Kolstrup Søgaard Nielsen, Leif Kofoed Nielsen, Lisa Leth Maroun, Morten Hanefeld Dziegiel, Lise Grupe Larsen, Lisbeth Ehlert Knudsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Validation of in vitro test systems using the modular approach with steps addressing reliability and relevance is an important aim when developing in vitro tests in e.g. reproductive toxicology. The ex vivo human placental perfusion system may be used for such validation, here presenting the placental perfusion model in Copenhagen including control substances. The positive control substance antipyrine shows no difference in transport regardless of perfusion media used or of terms of delivery (n=59, p<0.05). Negative control studies with FITC marked dextran correspond with leakage criteria (<3mlh -1 from the fetal reservoir) when adding 2 (n=7) and 20mg (n=9) FITC-dextran/100ml fetal perfusion media. Success rate of the Copenhagen placental perfusions is provided in this study, including considerations and quality control parameters. Three checkpoints suggested to determine success rate revealed that 15% of the cannulated placentae received in one year (n=202) were successfully perfused.

Original languageEnglish
JournalReproductive Toxicology
Volume30
Issue number1
Pages (from-to)138-146
Number of pages9
ISSN0890-6238
DOIs
Publication statusPublished - Aug 2010

Keywords

  • Animal Testing Alternatives
  • Antipyrine
  • Equipment Design
  • Female
  • Humans
  • In Vitro Techniques
  • Maternal-Fetal Exchange
  • Perfusion
  • Placenta
  • Pregnancy
  • Quality Control
  • Reproduction
  • Toxicity Tests
  • Journal Article
  • Research Support, Non-U.S. Gov't

Fingerprint

Dive into the research topics of 'Quality assessment of a placental perfusion protocol'. Together they form a unique fingerprint.

Cite this